BulletImage.pngHPLC eLearning Series

Advanced HPLC Method Development Using Quality by Design

This 38-part online course teaches you strategy and technique to develop high-quality HPLC and UHPLC methods quickly and with confidence. You will learn a time-proven technique that walks you through the method development process a step at a time using sound chromatographic principles to help make the process more efficient.....

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Contact Us

Please do contact me should you require an affordable quote for a number of users, multiple courses and or a site license for your colleagues.

Jackie Tan (jackie.tan@sepscience.com)

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What does it cover?

“Advanced HPLC Method Development Using Quality by Design” is an intensive online class that teaches you strategy and technique to develop high-quality HPLC and UHPLC methods quickly and with confidence. You will learn a time-proven technique that walks you through the method development process a step at a time using sound chromatographic principles to help make the process more efficient. The techniques can be used as a stand-alone strategy or added to existing development procedures to help streamline the process. Because the same principles apply to HPLC and UHPLC, you can apply the same information for the development of UHPLC methods.

The course covers:

  • What Quality by Design (QbD) is and how it applies to HPLC methods
  • A review of basic HPLC theory as it applies to method development
  • The makeup of HPLC columns including the support material and the bonded phase
  • The role of solvents in the separation
  • How the column plate number, selectivity, and retention can be used in a logical way to solve even the toughest separation problems.
  • How to use mobile phase solvents, pH, temperature, chemistry, and other variables to pull apart difficult-to-separate peak pairs.
  • How gradient elution works and how to remove the mystery of optimizing gradients.
  • How a scouting gradient can be used to speed the development of both isocratic and gradient methods.
  • How to use a simple spreadsheet calculator to move from the scouting run to the next step in method development
  • How computer-assisted method development tools can leverage a small amount of data into a large amount of information about a separation
  • How to take advantage of a publicly available internet database to identify equivalent and orthogonal columns
  • When standard reversed-phase techniques don’t work, how to choose mixed-mode, ion-pairing, normal phase, HILIC, chiral, or other chromatographic modes
    What’s the same and different when comparing HPLC and UHPLC
  • What to look for when validating a method

Price

This full course is available for 98 USD. You will have full access to the content for 6 months from purchase. You will be able to view the modules as many times as you like, providing not only a comprehensive introductory course to HPLC, but also a valuable reference tool to refresh you knowledge as and whn required.

Registration

To start your registration / purchase process simply complete the form opposite. Once you have completed the form it will be replaced with further instructions.

 
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