Principles of Size Exclusion Chromatography
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SEC is the simplest and mildest of all the liquid chromatography techniques and separates molecules on the basis of differences in size. This video describes the principles of the technique, and shows what happens inside the chromatography resin.
SEC for Protein Analysis
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This white paper presents fundamentals about analytical SEC and describes how our new-generation agarose-based SEC columns are used in analysis of biomolecules.
Whether you are a beginner or an expert, this handbook provide practical tips and in-depth information about size exclusion chromatography.
How does back pressure relate to resolution?
High resolution in HPLC is not achieved by applying back pressure to the column. Let’s straighten out what really helps you achieve high-resolution analytical SEC data.
Ensure SEC column reproducibility in protein drug analysis
Size exclusion chromatography (SEC) is the go-to analytical method in the analysis of therapeutic protein drugs. This article describes the factors affecting the reproducibility of columns and how you can minimize column variation.
Stop hoping for an OK resin bed—start looking into your HPLC column
Unless you have X-ray vision, it’s pretty hard to inspect your chromatography resin bed in a stainless steel HPLC column. Here’s how transparent columns can improve the performance of your analytical size exclusion chromatography (SEC) run.
Master analytical SEC running conditions
Need to find out how to calibrate your size exclusion chromatography (SEC) column? Or how different additives affect your results? Read these useful insights.
How to improve your SEC results
Sometimes your size exclusion chromatography (SEC) results don’t look like you want them to. Fix issues like poor resolution, peak tailing and fronting with the tips in this FAQ.