Separation Science, in collaboration with Agilent, offers an eBook focusing on high-throughput LC-MS applications of the RapidFire 400 system. This compendium features application notes covering method development, drug discovery, ADME, clinical research and forensics and toxicology, including:

METHOD DEVELOPMENT
High-Throughput (Sub-2.5 Second) Direct Injection Analysis by Mass Spectrometry
This application note presents a modified Agilent RapidFire high‑throughput MS system (RapidFire) that bypasses SPE desalting and enables a sub-2.5 second sample throughput rate, making the analysis of 35,000 samples a day possible by Q-TOF.

Automated Method Development Using the Agilent RapidFire High‑Throughput Mass Spectrometry System
This application note demonstrates a procedure for optimizing a method for a small example molecule, cyclic AMP. The final method has a CV of <3%, greatly improved peak shape, and 20-fold reduced carryover as compared to the generic starting method.

Minimization of the Required Sample Volume for Agilent RapidFire High-Throughput Mass Spectrometry Systems
This application note describes analysis with the Agilent RapidFire high-throughput MS system using tubing with a smaller inner diameter (id). This way, the necessary sample volume could be minimized.

DRUG DISCOVERY
Fragment-Based Drug Discovery: Comparing Labeled and Label-Free Screening
This application note screens β-amyloid secretase (BACE-1) against a fragment library using two substrates, a labeled and an unlabeled peptide, which were detected either by FS or ultrafast SPE/MS/MS using the Agilent RapidFire high-throughput MS system.

High-Throughput Lead Discovery with Agilent RapidFire/MS Systems
In this application note, a stearoyl-coenzyme A desaturase (SCD) assay is used to illustrate the power of Agilent RapidFire/MS systems for screening intractable targets.

ADME
Ultrafast Analysis of In Vitro Microsomal Metabolic Stability using RapidFire Coupled to the Ultivo Triple Quadrupole Mass Spectrometer
This study compared the results of in vitro microsomal metabolic stability (MMS) assays analyzed by RapidFire/TQ and LC/TQ.

CLINICAL RESEARCH
Ultrafast Analysis of Levetiracetam in Serum
This application note evaluates the ability of an ultrafast SPE/MS/MS system (Agilent RapidFire high-throughput MS system) which is capable of analysis times of <10 seconds per sample to analyze levetiracetam in human serum.

FORENSICS & TOXICOLOGY
Feasibility of the Agilent RapidFire High-Throughput MS System for Ultrafast Screening of Drug Targets
The Agilent RapidFire high-throughput MS system and Agilent 6545 LC/Q-TOF (RF/Q-TOF) have been used to develop an efficient, qualitative method for the simultaneous analysis of a subset of analytes currently screened by ELISA. This method uses a supported liquid extraction (SLE) before RF injection and Q-TOF auto-MS/MS data acquisition.