Separation Science, in collaboration with Shimadzu, offers an eBook containing a selection of application notes relevant for pharmaceutical and biopharmaceutical analysis using HPLC / (U)HPLC or LC-MS/MS. Discover an effective method for drug quantitation in biological fluids, a high-throughput analysis for simple fractionation, the aDownload the eBook ...
The eBook contains the following applications:
Ultra-Fast LC-MS/MS Analysis of Drugs in Biological FluidsThere is increasing demand in modern clinical/pharmaceutical laboratories for high-throughput liquid chromatography-tandem mass spectrometry analysis for drug quantitation in biological fluids. The analysis of drugs and metabolites using LC-MS/MS typically requires analysis times of a few minutes per sample. In this report, we describe the use of a high-speed autosampler for the precise and ultra-fast quantitation of drugs in plasma samples within 20 seconds, introducing real examples of increased productivity in the lab.
Learning Outcomes
- Ultrafast autosampler reduces overall analysis times for higher throughput
- Carryover is kept to an absolute minimum with active rinsing
High Efficiency in Workflow from Preparative HPLC to Analytical HPLC
Preparative LC is widely used as a technique for separation and purification of a target compound from a mixture. Identification of the target compound peak and confirmation of fraction purity are extremely time-consuming, and automation of these processes can be expected to shorten the total working time for purification process. This article introduces a high-throughput analysis conducted for simple fractionation using MS triggers and purity confirmation using an analytical/preparative LC-MS system.
Learning Outcomes
- Liquid handler is equipped with parallel preparative and analytical flow paths, enabling seamless re-analysis of the collected fractions
- Automated processes increase efficiency for the entire workflow, from fractionation to re-analysis
Application of a Dual Injection (U)HPLC for the Analysis of Pharmaceuticals
Many people pursue ideas of “efficiency” as an ideal for daily life; the same can be true in the HPLC laboratory. For instance, a (U)HPLC system with two injection ports (independent flow paths) allows two analyses to be run simultaneously. This note demonstrates the efficiency, throughput, and reliability of a dual injection system for finished pharmaceutical products and in-process active pharmaceutical ingredients. In addition, this work also shows the successful method transfer to superficially porous (SPP) columns for chromatographic analysis with isocratic elution.
Learning Outcomes
- Dual injection technique allows two analyses to be run simultaneously, doubling throughput
- Shows the successful method transfer to superficially porous (SPP) columns
Impurity Analysis in Pharmaceutical Products Using Diode Array Detection
The accurate determination of trace impurities is essential in both pharmaceutical drug development and drug manufacturing to meet ICH guidelines and ensure product safety. This application note demonstrates the capabilities of an advanced photodiode array detector, featuring a wide linearity range, low noise, and high sensitivity, for analyzing trace impurities in pharmaceuticals. Results show excellent linearity and informative spectra over a wide range of concentrations.
Learning Outcomes
- PDA detector eliminates the effects of stray light during detection
- Delivers outstanding performance over a wide range of concentrations
Comprehensive Fractionation of Herbal Medicine Components by PDA-ELSD Triggered Preparative LC
With preparative LC, an ultraviolet (UV) detector or a photodiode array detector (PDA) is commonly used. However, a preparative purification of all compounds without any omission is not possible because these detectors cannot detect non-UV absorbing compounds. This article introduces a comprehensive fractionation for both UV absorbing and non-UV absorbing compounds using a newly designed PDA-ELSD triggered preparative LC that equips an evaporative light scattering detector (ELSD) in addition to the PDA.
Learning Outcomes
- Comprehensive fractionation can be performed without missing non-UV absorbing compounds
- Graphical user interface provides easy and labor-saving operation for fractionation settings
- Smooth transfer from preliminary analytical LC to preparative purification LC is possible
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