Functional nucleic acids, beginning with nucleic acid drugs, have attracted considerable attention in recent years. In the development and evaluation of these functional nucleic acids, it is essential to understand their thermal stability, as this is a factor that controls their structure and functions. Thermal stability analysis is also indispensable in techniques based on hybridization of nucleic acids such as PCR and DNA microarray.

Oligonucleotide therapeutics are synthetic oligonucleotides that demonstrate medical efficacy by bonding with target genes or target proteins that cause various diseases.

The series of application notes below provide solutions for oligonucleotide drug analysis. Simply complete the form to download your free copies.


Thermal Stability Analysis of Nucleic Acid Drugs by TMSPC™-8 Tm Analysis System
Shimadzu_App1Thermal stability analysis (Tm analysis) of a nucleic acid was conducted using a Shimadzu UV-1900i ultraviolet-visible (UV-Vis) spectrophotometer and TMSPC-8 Tm analysis system. This system provides a simple method to calculate the Tm value by measuring the absorbance transition at 260 nm at increasing temperatures.

Learning outcome:
Discover how the TMSPC-8 Tm analysis system can simplify thermal stability analysis of nucleotides.


Synthesis Confirmation for Nucleic Acid Medicines - Rapid Sequence Confirmation Using a MALDI-TOF Mass Spectrometer
Shimadzu_App2This application shows how the molecular weight and base sequence of a synthetic nucleic acid can be determined using MALDI-TOF mass spectrometry.

Learning Outcome:
See how Shimadzu’s benchtop MALDI-8020 mass spectrometer makes it easy to confirm the molecular weight and base sequence of nucleic acids.


Verification of Quantitativity and Confirmation of Molecular Weight of Oligonucleotide Therapeutics Using LCMS™-8060
Shimadzu_App3Here we introduce the analysis of a 20 base 2’-MOE modified oligonucleotide using an LCMS-8060 triple quadrupole mass spectrometer. A calibration curve for 1 to 300 ng/mL was obtained, and the molecular weight was confirmed within a small error range by deconvolution function from the scan data.

Learning Outcome:
Learn how Shimadzu’s LCMS-8060 can be used to determine the molecular weight of synthetic oligonucleotides.


Determination of Molecular Mass and Quantification of Oligonucleotide Therapeutics Using Quadrupole Time-of-Flight Mass Spectrometer LCMS™-9030
Shimadzu_App4This application shows an example analysis of a 20 base 2’-MOE modified oligonucleotide using the LCMS-9030 Q-TOF mass spectrometer. The molecular mass was determined with an error of just 0.05 ppm. A calibration curve was prepared using the MRM mode, demonstrating linearity within a range of 1–1000 ng/mL.

Learning outcome:
Learn how the accurate mass measurement of Shimadzu’s LCMS-9030 can be used to identify the structure of oligonucleotide therapeutics.

Complete the form on the right to download your free copies of these application notes.