Bioanalytical LC/MS: Fundamentals and Applications

FredKlink_75.pngWhen: 30-31 October, 2017
Duration: 2 Days
Presenter: Frederick Klink

EARLY BIRD RATE (ends 31 August)
USD 975 (standard rate - USD 1,175)

This two-day, comprehensive overview course provides attendees with relevant, practical approaches that can be immediately applied in the lab. The theory of mass spectrometry and HPLC interfaces is presented to the extent necessary to understand operation of instruments but the real focus of this class is improving your ability to use LC/MS on a daily basis for various types of biological samples.

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Who Should Attend
Researchers, practitioners, technicians, and others who are currently using LC, LC/MS, LC/MS/MS, or plan to use LC/MS in the future, and those dealing with data produced by LC/MS. This course is designed to provide a practical overview. Chromatographers just embarking on the technique will gain insight on how to select the appropriate instrument for different applications, and those currently using LC/MS and its data will develop an appreciation for, and an understanding of, the complexities of the data generated.

Course Topics

  • Introduction to LC/MS: overview and historical perspective
  • What is MS?: mass analyzer types, MS/MS and CAD
  • LC/MS Interfaces: atmospheric pressure chemical ionization, electrospray and atmospheric pressure photoionization interfaces
  • Optimization of LC/MS methods: how mobile phase and matrix components can affect results; methods for determining and fixing optimization problems; how electrospray method development differs from APCI
  • Handling the data from LC/MS: both qualitative (spectral interpretation) and quantitative use of MS data is discussed
  • Applications review: drugs and metabolites, pharmaceuticals, natural products, protein/peptide analyses

How You'll Benefit from This Course

  • Understand the operational fundamentals of liquid chromatography, mass spectrometry, and the commercially available LC/MS interfaces.
  • Receive an overview of LC/MS applications for bioanalysis in pharmaceutical and biotechnology industries plus information on other commercial and research applications.
  • Learn how to modify existing HPLC methods to optimize results when moving to LC/MS.
  • Find out how to use LC/MS to obtain qualitative information about your samples; simple molecular weight determinations; isotope ratio calculation for elemental composition; structural analysis through spectral interpretation; peptide sequencing with MS/MS.
  • Learn what method development procedures and system suitability tests are necessary for use of
  • LC/MS in a regulated environment such as pharmaceutical manufacturing.

The Presenter
Fred Klink is the only trainer in this field who has (1) compiled all of the important aspects of methods development into one, integrated, step-wise approach and (2) incorporated concepts of quality, validation, and regulatory compliance into his method development scheme. This latter aspect is, of course, of vital importance in the pharmaceutical and biotech industries but is also of value to anyone who wants to develop rugged, reproducible, transferable methods.
In addition to methods development, Fred’s specialty areas are HPLC and SPE technologies and the application of mass spectrometry to protein characterization. Fred is the author of several journal articles and two book chapters: one for the Encyclopedia of Analytical Chemistry (Wiley, 2010) and another for Basic HPLC and CE of Biomolecules (BBL, 1998).

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