An introduction to the use of LC-MS/MS, with an emphasis on the analysis of drugs in biological matrices. You will learn how a mass spectrometer works, the advantages (and limitations) of adding mass spectrometric detection to your liquid chromatographic analyses, and how to use MS to approach separation and detection problems intractable by other methods.
With the introduction of the mass spectrometer (MS) as a practical detector for a high-performance liquidchromatograph (LC or HPLC) in the early 1990s, LC-MS began to be used for routine applications. One form of LC-MS uses a tandem MS (LC-MS/MS), that has become the go-to instrument for quantitative analysis of drugs inbiological matrices in the pharmaceutical laboratory. As they have become more widely used and understood,LC-MS and LC-MS/MS have found their way into environmental applications, synthetic chemistry, protein analysis,and many other areas. LC-MS/MS has moved from a technique available only to skilled experts in both HPLC andMS to a routine tool usable much as any other HPLC detector.
You will find that this introductory course in LC-MS/MS will give you a good foundation of understanding the technique and how to use it in your daily work. We start out with an overview of different MS techniques, and focus in the majority of the course on the quadrupole mass spectrometer. You will learn how MS works, how ionsare produced and fragmented. Sections focus on qualitative, as well as quantitative analysis. Each part of the process, calibration, tuning, sample preparation, HPLC method development, and MS method development are covered. Many tricks and techniques of practical use are presented.
You will learn how a mass spectrometer works, the advantages (and limitations) of adding mass spectrometric detection to your liquid chromatographic analyses, and how to use MS to approach separation and detection problems intractable by other methods. By understanding the central roles of the LC-MS interface and the sample ion, you will have a solid grounding on which to further build your LC-MS expertise.
This full course is available for 98 USD. You will have full access to the content for 6 months from purchase. You will be able to view the modules as many times as you like, providing not only a comprehensive introductory course to HPLC, but also a valuable reference tool to refresh you knowledge as and whn required.
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