--- title: Protein Analysis with Size Exclusion Chromatography Portal Applications --- [Chromatography Forum](http://www.chromforum.org/) [Analytical Training Solutions](https://www.analytical-training-solutions.com) [日本語](https://www.sepscience.com/japan) [![Separation Science](https://learning.sepscience.com/hubfs/Sepscience_Dec2016/images/-resized-strch-SepSci_WebHeader_v3.png)](https://www.sepscience.com/) [Chromatography Forum](http://www.chromforum.org/) [Analytical Training Solutions](https://www.analytical-training-solutions.com) [日本語](https://blog.sepscience.com/japan) [Sign Up for Newsletter](https://www.sepscience.com/subscribe/) ![MicrositeHeader_v3.png](https://learning.sepscience.com/hs-fs/hubfs/Companies/GE%20Healthcare/Microsite/MicrositeHeader_v3.png?width=1139&height=169&name=MicrositeHeader_v3.png) Size exclusion chromatography (SEC) is currently the most powerful chromatography technique for obtaining reliable information about the size of biomolecules under native conditions. As such, it is widely used in several different analytical applications from basic research to quality control of biotherapeutics. This educational microsite, produced in collaboration with GE Healthcare, presents fundamentals about analytical SEC, tips to ensure successful analysis, available products and applications showcasing the role of SEC in biomolecule analysis. [![Home](https://no-cache.hubspot.com/cta/default/2322077/ad468a9c-981c-4e03-bd24-61f181f67e05.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/ad468a9c-981c-4e03-bd24-61f181f67e05)   [![SEC Tips & Fundamentals](https://no-cache.hubspot.com/cta/default/2322077/f4dfaac0-9043-4709-a49e-667a21857235.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/f4dfaac0-9043-4709-a49e-667a21857235)   [![SEC Columns](https://no-cache.hubspot.com/cta/default/2322077/18fd503b-343b-4d54-95cb-ec4484ad388d.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/18fd503b-343b-4d54-95cb-ec4484ad388d)   [![Applications](https://no-cache.hubspot.com/cta/default/2322077/8bd731a3-833e-42f8-8e2c-53fab82330c3.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/8bd731a3-833e-42f8-8e2c-53fab82330c3)   [![Sample Prep Tips](https://no-cache.hubspot.com/cta/default/2322077/c573622b-fc10-4784-a368-fae6fa75194a.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/c573622b-fc10-4784-a368-fae6fa75194a)   [![FAQs](https://no-cache.hubspot.com/cta/default/2322077/94f385d3-2f3b-4306-b77b-3e9a0a5291bc.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/94f385d3-2f3b-4306-b77b-3e9a0a5291bc)   [![Contact](https://no-cache.hubspot.com/cta/default/2322077/bb032dc3-f1e9-4afc-9ca6-abe29923377f.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/bb032dc3-f1e9-4afc-9ca6-abe29923377f) ## Applications #### Analysis of membrane proteins using size exclusion chromatography SEC has shown to be a versatile tool for use in a broad range of applications. As buffer conditions do not affect separation, SEC is especially suited for applications including membrane proteins that are difficult to study due to their hydrophobic, poorly soluble nature. [![Learn more >>](https://no-cache.hubspot.com/cta/default/2322077/1ea15e8e-dc6e-4c6d-9296-dd81a7f4c935.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/1ea15e8e-dc6e-4c6d-9296-dd81a7f4c935) #### ![MemConfChanges.png](https://learning.sepscience.com/hs-fs/hubfs/Companies/GE%20Healthcare/Microsite/MemConfChanges.png?width=120&height=154&name=MemConfChanges.png)Analysis of conformational changes of a membrane protein using size exclusion chromatography This application note describes the use of Superdex™ 200 Increase 5/150 GL column for size in the analysis of conformational changes of the CorA magnesium channel in response to Mg2+ binding. SEC allowed for rapid analysis of protein complex formation under non-denaturing conditions. By simply altering buffer conditions, Mg2+-dependent conformational changes could be studied with 20 mutants in a single 24 h experiment. [![Download application note >>](https://no-cache.hubspot.com/cta/default/2322077/4d53f083-a08c-45b3-b331-56cb8e039164.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/4d53f083-a08c-45b3-b331-56cb8e039164) #### ![AppScaleUpdab.png](https://learning.sepscience.com/hs-fs/hubfs/Companies/GE%20Healthcare/Microsite/AppScaleUpdab.png?width=120&height=151&name=AppScaleUpdab.png)Scale-up and process economy calculations of a dAb purification process using ready-to-use products Purification of a domain antibody (dAb) in a chromatography capture step was successfully scaled from laboratory to pilot scale using ready-to-use equipment and ready-made buffers. In this study Superdex 75 Increase 10/300 GL column was used to analyze dAb purity by SEC. [![Download application note >>](https://no-cache.hubspot.com/cta/default/2322077/a1842835-55a0-480e-912d-5d409f3e0a7a.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/a1842835-55a0-480e-912d-5d409f3e0a7a) #### ![AppMonClon200.png](https://learning.sepscience.com/hs-fs/hubfs/Companies/GE%20Healthcare/Microsite/AppMonClon200.png?width=120&height=149&name=AppMonClon200.png)Size exclusion chromatography analysis of papain-cleaved monoclonal antibody using Superdex 200 Increase columns This application note describes a simplified workflow for the production of Fab (fragment, antigen binding). Intermediates and end products were analyzed with SEC on two different sized Superdex 200 Increase columns: one 300 mm and one 150 mm in length. The high resolution power of Superdex 200 Increase allows baseline separation between antibody monomer, aggregates, and fragments. [![Download application note >>](https://no-cache.hubspot.com/cta/default/2322077/5cd19db7-e7a1-479d-a61a-e500cc09fcab.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/5cd19db7-e7a1-479d-a61a-e500cc09fcab) Eclipse Business Media Ltd, Regd in England, No. 06513189, Woodview, Bull Lane Industrial Estate, Sudbury, CO10 0FD, United Kingdom, T +44 (0)161 818 7434, [info@sepscience.com](mailto:info@sepscience.com)   Eclipse Business Media Ltd needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our [Privacy Policy](https://www.sepscience.com/privacy-policy/) or contact [privacy@labxmediagroup.com](mailto:privacy@labxmediagroup.com)  Copyright © 2025. 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