Analysis of membrane proteins using size exclusion chromatography

SEC has shown to be a versatile tool for use in a broad range of applications. As buffer conditions do not affect separation, SEC is especially suited for applications including membrane proteins that are difficult to study due to their hydrophobic, poorly soluble nature.

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MemConfChanges.pngAnalysis of conformational changes of a membrane protein using size exclusion chromatography

This application note describes the use of Superdex™ 200 Increase 5/150 GL column for size in the analysis of conformational changes of the CorA magnesium channel in response to Mg2+ binding. SEC allowed for rapid analysis of protein complex formation under non-denaturing conditions. By simply altering buffer conditions, Mg2+-dependent conformational changes could be studied with 20 mutants in a single 24 h experiment.

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AppScaleUpdab.pngScale-up and process economy calculations of a dAb purification process using ready-to-use products

Purification of a domain antibody (dAb) in a chromatography capture step was successfully scaled from laboratory to pilot scale using ready-to-use equipment and ready-made buffers. In this study Superdex 75 Increase 10/300 GL column was used to analyze dAb purity by SEC.

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AppMonClon200.pngSize exclusion chromatography analysis of papain-cleaved monoclonal antibody using Superdex 200 Increase columns

This application note describes a simplified workflow for the production of Fab (fragment, antigen binding). Intermediates and end products were analyzed with SEC on two different sized Superdex 200 Increase columns: one 300 mm and one 150 mm in length. The high resolution power of Superdex 200 Increase allows baseline separation between antibody monomer, aggregates, and fragments.

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