SEC for Protein Analysis

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This white paper presents fundamentals about analytical SEC and describes how our new-generation agarose-based SEC columns are used in analysis of biomolecules.


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Analytical SEC

How does back pressure relate to resolution?

High resolution in HPLC is not achieved by applying back pressure to the column. Let’s straighten out what really helps you achieve high-resolution analytical SEC data.


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Membrane Proteins

The benefits of SEC

SEC has shown to be a versatile tool for use in a broad range of applications. As buffer conditions do not affect separation, SEC is especially suited for applications including membrane proteins that are difficult to study due to their hydrophobic, poorly soluble nature.

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Column Reproducibility

Ensure SEC column reproducibility in protein drug analysis

Size exclusion chromatography (SEC) is the go-to analytical method in the analysis of therapeutic protein drugs. This article describes the factors affecting the reproducibility of columns and how you can minimize column variation.

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Principles of Size Exclusion Chromatography

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SEC is the simplest and mildest of all the liquid chromatography techniques and separates molecules on the basis of differences in size. This video describes the principles of the technique, and shows what happens inside the chromatography resin.

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