The benefits of SEC
SEC has shown to be a versatile tool for use in a broad range of applications. As buffer conditions do not affect separation, SEC is especially suited for applications including membrane proteins that are difficult to study due to their hydrophobic, poorly soluble nature.
Principles of Size Exclusion Chromatography
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SEC is the simplest and mildest of all the liquid chromatography techniques and separates molecules on the basis of differences in size. This video describes the principles of the technique, and shows what happens inside the chromatography resin.