--- title: Protein Analysis with Size Exclusion Chromatography Portal Questions --- [Chromatography Forum](http://www.chromforum.org/) [Analytical Training Solutions](https://www.analytical-training-solutions.com) [日本語](https://www.sepscience.com/japan) [![Separation Science](https://learning.sepscience.com/hubfs/Sepscience_Dec2016/images/-resized-strch-SepSci_WebHeader_v3.png)](https://www.sepscience.com/) [Chromatography Forum](http://www.chromforum.org/) [Analytical Training Solutions](https://www.analytical-training-solutions.com) [日本語](https://blog.sepscience.com/japan) [Sign Up for Newsletter](https://www.sepscience.com/subscribe/) ![MicrositeHeader_v3.png](https://learning.sepscience.com/hs-fs/hubfs/Companies/GE%20Healthcare/Microsite/MicrositeHeader_v3.png?width=1139&height=169&name=MicrositeHeader_v3.png) Size exclusion chromatography (SEC) is currently the most powerful chromatography technique for obtaining reliable information about the size of biomolecules under native conditions. As such, it is widely used in several different analytical applications from basic research to quality control of biotherapeutics. This educational microsite, produced in collaboration with GE Healthcare, presents fundamentals about analytical SEC, tips to ensure successful analysis, available products and applications showcasing the role of SEC in biomolecule analysis. [![Home](https://no-cache.hubspot.com/cta/default/2322077/ad468a9c-981c-4e03-bd24-61f181f67e05.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/ad468a9c-981c-4e03-bd24-61f181f67e05)   [![SEC Tips & Fundamentals](https://no-cache.hubspot.com/cta/default/2322077/f4dfaac0-9043-4709-a49e-667a21857235.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/f4dfaac0-9043-4709-a49e-667a21857235)   [![SEC Columns](https://no-cache.hubspot.com/cta/default/2322077/18fd503b-343b-4d54-95cb-ec4484ad388d.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/18fd503b-343b-4d54-95cb-ec4484ad388d)   [![Applications](https://no-cache.hubspot.com/cta/default/2322077/9df0e48c-317b-4e1d-b8a8-b28628c9fbd3.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/9df0e48c-317b-4e1d-b8a8-b28628c9fbd3)   [![Sample Prep Tips](https://no-cache.hubspot.com/cta/default/2322077/c573622b-fc10-4784-a368-fae6fa75194a.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/c573622b-fc10-4784-a368-fae6fa75194a)   [![FAQs](https://no-cache.hubspot.com/cta/default/2322077/ae00ec87-def1-428c-bef9-17698c2fd6e9.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/ae00ec87-def1-428c-bef9-17698c2fd6e9)   [![Contact](https://no-cache.hubspot.com/cta/default/2322077/bb032dc3-f1e9-4afc-9ca6-abe29923377f.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/bb032dc3-f1e9-4afc-9ca6-abe29923377f) ## Your Questions Answered... During a webinar titled 'Protein Characterization using Size Exclusion Chromatography' we organized a live question and answer session that generated more than 90 questions. Some of these questions have been compiled into a PDF document that you can download by clicking on the button below... [![FAQs Download >>](https://no-cache.hubspot.com/cta/default/2322077/69d6d8f2-7aec-4d01-a271-0cae13958ad1.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/69d6d8f2-7aec-4d01-a271-0cae13958ad1)   ## Discussion Forum Join this discussion forum to share and ask questions related to protein analysis... [![Join the discussion >>](https://no-cache.hubspot.com/cta/default/2322077/ec608904-8188-413d-adbd-18210673659c.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/ec608904-8188-413d-adbd-18210673659c)     ## More of Your Questions... You asked us about analytical SEC quality certificates, compatibility with HPLC systems, considerations for column selection, and more. Here are the answers. [![See your questions answered >>](https://no-cache.hubspot.com/cta/default/2322077/f41f5181-032c-47ca-985a-936b61303d31.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/f41f5181-032c-47ca-985a-936b61303d31)   [![Download Q&A Book](https://no-cache.hubspot.com/cta/default/2322077/9848c99f-5583-4948-9bd4-31c4695639db.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/9848c99f-5583-4948-9bd4-31c4695639db) Eclipse Business Media Ltd, Regd in England, No. 06513189, Woodview, Bull Lane Industrial Estate, Sudbury, CO10 0FD, United Kingdom, T +44 (0)161 818 7434, [info@sepscience.com](mailto:info@sepscience.com)   Eclipse Business Media Ltd needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. 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