--- title: Protein Analysis with Size Exclusion Chromatography Portal SEC Tips --- [Chromatography Forum](http://www.chromforum.org/) [Analytical Training Solutions](https://www.analytical-training-solutions.com) [日本語](https://www.sepscience.com/japan) [![Separation Science](https://learning.sepscience.com/hubfs/Sepscience_Dec2016/images/-resized-strch-SepSci_WebHeader_v3.png)](https://www.sepscience.com/) [Chromatography Forum](http://www.chromforum.org/) [Analytical Training Solutions](https://www.analytical-training-solutions.com) [日本語](https://blog.sepscience.com/japan) [Sign Up for Newsletter](https://www.sepscience.com/subscribe/) ![MicrositeHeader_v3.png](https://learning.sepscience.com/hs-fs/hubfs/Companies/GE%20Healthcare/Microsite/MicrositeHeader_v3.png?width=1139&height=169&name=MicrositeHeader_v3.png) Size exclusion chromatography (SEC) is currently the most powerful chromatography technique for obtaining reliable information about the size of biomolecules under native conditions. As such, it is widely used in several different analytical applications from basic research to quality control of biotherapeutics. This educational microsite, produced in collaboration with GE Healthcare, presents fundamentals about analytical SEC, tips to ensure successful analysis, available products and applications showcasing the role of SEC in biomolecule analysis. [![Home](https://no-cache.hubspot.com/cta/default/2322077/ad468a9c-981c-4e03-bd24-61f181f67e05.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/ad468a9c-981c-4e03-bd24-61f181f67e05)   [![SEC Tips & Fundamentals](https://no-cache.hubspot.com/cta/default/2322077/f4dfaac0-9043-4709-a49e-667a21857235.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/f4dfaac0-9043-4709-a49e-667a21857235)   [![SEC Columns](https://no-cache.hubspot.com/cta/default/2322077/18fd503b-343b-4d54-95cb-ec4484ad388d.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/18fd503b-343b-4d54-95cb-ec4484ad388d)   [![Applications](https://no-cache.hubspot.com/cta/default/2322077/9df0e48c-317b-4e1d-b8a8-b28628c9fbd3.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/9df0e48c-317b-4e1d-b8a8-b28628c9fbd3)   [![Sample Prep Tips](https://no-cache.hubspot.com/cta/default/2322077/d9b5e739-5c9d-4038-b8f7-8a4aa1bc8eaf.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/d9b5e739-5c9d-4038-b8f7-8a4aa1bc8eaf)   [![FAQs](https://no-cache.hubspot.com/cta/default/2322077/94f385d3-2f3b-4306-b77b-3e9a0a5291bc.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/94f385d3-2f3b-4306-b77b-3e9a0a5291bc)   [![Contact](https://no-cache.hubspot.com/cta/default/2322077/bb032dc3-f1e9-4afc-9ca6-abe29923377f.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/bb032dc3-f1e9-4afc-9ca6-abe29923377f) ## Regenerated cellulose in HPLC filters #### Broad compatibility increases lab consistency... HPLC is a versatile technique for chemical analysis that employs a range of different solvents depending on the application. The technique benefits from filtration during sample preparation to remove particulates. Regenerated cellulose consists of pure cellulose without the addition of any wetting agent. After solubilization and casting, it results in a uniform, reproducible membrane for filtration.   [![Learn more >>](https://no-cache.hubspot.com/cta/default/2322077/adc46be9-1a28-4895-9da4-18959d4857c2.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/adc46be9-1a28-4895-9da4-18959d4857c2)    ## Do's and don'ts when filtering viscous liquids #### Save time and boost lab efficiency with this guide... If you work in an analytical lab that performs filtration prior to analysis, then you’re probably used to processing many samples under a set time constraint. Any way to save time in analytical sample preparation while still delivering accurate, consistent results can help get products released quickly and safely.   [![Learn more >>](https://no-cache.hubspot.com/cta/default/2322077/a2003e91-3fe6-489e-bb32-d6a532a942e5.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/a2003e91-3fe6-489e-bb32-d6a532a942e5)     ## Save time in HPLC prep #### Using multilayer and all-in-one filter units... If you analyse large numbers of samples using HPLC, sample preparation can take up a lot of your time. Filtering samples before HPLC can help avoid frit clogging and while maintaining data quality. So, what can you do to simplify and speed up the process?   [![Learn more >>](https://no-cache.hubspot.com/cta/default/2322077/f349264a-202e-4778-a404-88c7a8e84c5f.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/f349264a-202e-4778-a404-88c7a8e84c5f)   ## HPLC Pain Points – Part 1 #### Avoid Pitfalls in HPLC Sample Prep Ensuring that systems are in good working order minimizes equipment downtime and increases lab efficiency. Problems in an HPLC system can arise from several sources. The source of a given problem can often be identified by looking at the system itself or by checking downstream, by looking at the chromatogram. [![Learn more >>](https://no-cache.hubspot.com/cta/default/2322077/08f3d339-412c-49aa-bed2-606f88bbc4ae.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/08f3d339-412c-49aa-bed2-606f88bbc4ae)     ## HPLC Pain Points – Part 2 #### Troubleshooting Strategies Maintaining an HPLC system in good condition provides users with consistent data quality and accuracy. A well-functioning system also minimizes downtime, helping to maintain lab efficiency. When using a UV detector, the chromatogram can indicate the presence of a problem and provides clues about where to find the cause. [![Learn more >>](https://no-cache.hubspot.com/cta/default/2322077/2bf4e25b-89f2-41f0-a858-d3f272c23b77.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/2bf4e25b-89f2-41f0-a858-d3f272c23b77)   Eclipse Business Media Ltd, Regd in England, No. 06513189, Woodview, Bull Lane Industrial Estate, Sudbury, CO10 0FD, United Kingdom, T +44 (0)161 818 7434, [info@sepscience.com](mailto:info@sepscience.com)   Eclipse Business Media Ltd needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our [Privacy Policy](https://www.sepscience.com/privacy-policy/) or contact [privacy@labxmediagroup.com](mailto:privacy@labxmediagroup.com)  Copyright © 2025. 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