--- title: Protein Analysis with Size Exclusion Chromatography Portal SEC Columns --- [Chromatography Forum](http://www.chromforum.org/) [Analytical Training Solutions](https://www.analytical-training-solutions.com) [日本語](https://www.sepscience.com/japan) [![Separation Science](https://learning.sepscience.com/hubfs/Sepscience_Dec2016/images/-resized-strch-SepSci_WebHeader_v3.png)](https://www.sepscience.com/) [Chromatography Forum](http://www.chromforum.org/) [Analytical Training Solutions](https://www.analytical-training-solutions.com) [日本語](https://blog.sepscience.com/japan) [Sign Up for Newsletter](https://www.sepscience.com/subscribe/) ![MicrositeHeader_v3.png](https://learning.sepscience.com/hs-fs/hubfs/Companies/GE%20Healthcare/Microsite/MicrositeHeader_v3.png?width=1139&height=169&name=MicrositeHeader_v3.png) Size exclusion chromatography (SEC) is currently the most powerful chromatography technique for obtaining reliable information about the size of biomolecules under native conditions. As such, it is widely used in several different analytical applications from basic research to quality control of biotherapeutics. This educational microsite, produced in collaboration with GE Healthcare, presents fundamentals about analytical SEC, tips to ensure successful analysis, available products and applications showcasing the role of SEC in biomolecule analysis. [![Home](https://no-cache.hubspot.com/cta/default/2322077/ad468a9c-981c-4e03-bd24-61f181f67e05.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/ad468a9c-981c-4e03-bd24-61f181f67e05)   [![SEC Tips & Fundamentals](https://no-cache.hubspot.com/cta/default/2322077/f4dfaac0-9043-4709-a49e-667a21857235.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/f4dfaac0-9043-4709-a49e-667a21857235)   [![SEC Columns](https://no-cache.hubspot.com/cta/default/2322077/256dd876-df2b-49d0-8239-16d20490e012.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/256dd876-df2b-49d0-8239-16d20490e012)   [![Applications](https://no-cache.hubspot.com/cta/default/2322077/9df0e48c-317b-4e1d-b8a8-b28628c9fbd3.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/9df0e48c-317b-4e1d-b8a8-b28628c9fbd3)   [![Sample Prep Tips](https://no-cache.hubspot.com/cta/default/2322077/c573622b-fc10-4784-a368-fae6fa75194a.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/c573622b-fc10-4784-a368-fae6fa75194a)   [![FAQs](https://no-cache.hubspot.com/cta/default/2322077/94f385d3-2f3b-4306-b77b-3e9a0a5291bc.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/94f385d3-2f3b-4306-b77b-3e9a0a5291bc)   [![Contact](https://no-cache.hubspot.com/cta/default/2322077/bb032dc3-f1e9-4afc-9ca6-abe29923377f.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/bb032dc3-f1e9-4afc-9ca6-abe29923377f) ## Which Resin? Four choices of new generation agarose-based SEC resins for analysis of biomolecules. The prepacked columns offer rapid separations with high resolution for a variety of applications including aggregate analysis, studies of complex formation, and screening of samples. ![Resins_v2.png](https://learning.sepscience.com/hs-fs/hubfs/Companies/GE%20Healthcare/Microsite/Resins_v2.png?width=680&name=Resins_v2.png "Resins_v2.png")   ## Data & Applications #### ![Superdex30.png](https://learning.sepscience.com/hs-fs/hubfs/Companies/GE%20Healthcare/Microsite/Superdex30.png?width=70&name=Superdex30.png "Superdex30.png")Superdex™ 30 Increase Superdex 30 Increase prepacked columns are designed for rapid separation and analysis of peptides and other small biomolecules with molecular weights (Mr) ranging from ~ 100 to 7000. [![Learn more >>](https://no-cache.hubspot.com/cta/default/2322077/d1a4c17c-5d1c-4bb7-8cba-aef11755845f.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/d1a4c17c-5d1c-4bb7-8cba-aef11755845f)     #### ![Superdex75.png](https://learning.sepscience.com/hs-fs/hubfs/Companies/GE%20Healthcare/Microsite/Superdex75.png?width=70&name=Superdex75.png "Superdex75.png") #### Superdex 75 Increase Superdex 75 Increase prepacked columns are designed for rapid separation and analysis of proteins with Mr ranging from 3000 to 70 000. [![Learn more >>](https://no-cache.hubspot.com/cta/default/2322077/4f7270c7-9e35-4616-82a6-23bc8a646f06.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/4f7270c7-9e35-4616-82a6-23bc8a646f06)     #### ![Superdex200_v2.png](https://learning.sepscience.com/hs-fs/hubfs/Companies/GE%20Healthcare/Microsite/Superdex200_v2.png?width=70&name=Superdex200_v2.png "Superdex200_v2.png")Superdex 200 Increase Superdex 200 Increase prepacked columns are designed for rapid separation and analysis of proteins with Mr ranging from 10 000 to 600 000. [![Learn more >>](https://no-cache.hubspot.com/cta/default/2322077/ca661db1-a65e-456d-865b-5a4023f9901e.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/ca661db1-a65e-456d-865b-5a4023f9901e)     #### ![Superose6_v2.png](https://learning.sepscience.com/hs-fs/hubfs/Companies/GE%20Healthcare/Microsite/Superose6_v2.png?width=70&name=Superose6_v2.png "Superose6_v2.png")Superose 6 Increase Superose 6 Increase prepacked columns are designed for rapid separation and analysis of proteins and other biomolecules in the Mr range for globular proteins between 5000 and 5 000 000. This very wide fractionation range makes it suitable as a screening tool to explore the molecular-weight distribution of unknown samples. [![Learn more >>](https://no-cache.hubspot.com/cta/default/2322077/2d0cf574-3274-454a-a8ab-d64ccb3c4e11.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/2d0cf574-3274-454a-a8ab-d64ccb3c4e11)   ## Learn More & Order Whether you would like to order a column or to get additional information, visit the specific web pages... - [**Superdex 30 Increase**](https://www.gelifesciences.com/shop/chromatography/prepacked-columns/size-exclusion/superdex-30-increase-p-09431) - [**Superdex 75 Increase**](https://www.gelifesciences.com/shop/chromatography/prepacked-columns/size-exclusion/superdex-75-increase-p-06188) - [**Superdex 200 Increase**](https://www.gelifesciences.com/shop/chromatography/prepacked-columns/size-exclusion/superdex-200-increase-small-scale-size-exclusion-chromatography-columns-p-06190) - [**Superose 6 Increase**](https://www.gelifesciences.com/shop/chromatography/prepacked-columns/size-exclusion/superose-6-increase-small-scale-size-exclusion-chromatography-columns-p-05803) ...or complete the form below to discuss your column requirements with a GE Healthcare representative: Eclipse Business Media Ltd, Regd in England, No. 06513189, Woodview, Bull Lane Industrial Estate, Sudbury, CO10 0FD, United Kingdom, T +44 (0)161 818 7434, [info@sepscience.com](mailto:info@sepscience.com)   Eclipse Business Media Ltd needs the contact information you provide to us to contact you about our products and services. 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