--- title: Tosoh Bioscience Learning Portal description: Biopharmaceutical Application Notebook for the (U)HPLC analysis of biomolecules --- - [Home](https://www.sepscience.com/) - [Blog](https://blog.sepscience.com/) - [日本語](https://www.sepscience.com/japan) - [Chromatography Forum](http://www.chromforum.org/) - [Analytical Training Solutions](https://www.analytical-training-solutions.com) - [Contact Us](https://www.sepscience.com/eclipse-business-media/contact-us/) - [Subscribe for free](https://www.sepscience.com/subscribe/) [![Separation Science](https://learning.sepscience.com/hubfs/Sepscience_Dec2016/images/-resized-strch-SepSci_WebHeader_v3.png)](https://www.sepscience.com/) - [Food](https://www.sepscience.com/food) - [Enviro](https://www.sepscience.com/enviro) - [Pharma](https://www.sepscience.com/pharma) - [Bio Clinical](https://www.sepscience.com/bioclinical) - [LC](https://www.sepscience.com/lc) - [GC](https://www.sepscience.com/gc) - [MS](https://www.sepscience.com/ms) - [Methods & Applications](https://www.sepscience.com/methods-and-applications/) - [Webinars & eSeminars](https://www.sepscience.com/webinars-and-eseminars/) - [Videos & Tutorials](https://www.sepscience.com/videos-and-tutorials/) - [Home](https://www.sepscience.com/) - [Get in Touch with Us](https://www.sepscience.com/) ![Header_portal.png](https://learning.sepscience.com/hs-fs/hubfs/Learning%20Portals/Tosoh/Header_portal.png?width=1200&name=Header_portal.png "Header_portal.png") ### Thank you for visiting the Tosoh Bioscience Learning Portal Tosoh Bioscience has produced Volume 1 of its '*Biopharmaceutical Application Notebook*' for the (U)HPLC analysis of biomolecules. This essential application notebook will keep you informed of all the latest developments in the biopharmaceutical industry using both high performance liquid chromatography and ultra-high performance liquid chromatography — the analytical workhorses of the pharmaceutical industry. Here you will find a selection of the application notes covered in the '*Biopharmaceutical Application Notebook*'. To download the full copy, click below. [![Download Biopharmaceutical Application Notebook >>](https://no-cache.hubspot.com/cta/default/2322077/ece626e5-3a70-4e09-b26b-2a667b4b0694.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/ece626e5-3a70-4e09-b26b-2a667b4b0694)   ### ANTIBODIES Application Note High Speed and Resolution Analysis of mAbs by SEC ![Tosoh_App1.png](https://learning.sepscience.com/hs-fs/hubfs/Learning%20Portals/Tosoh/Tosoh_App1.png?width=120&name=Tosoh_App1.png "Tosoh_App1.png")Size exclusion chromatography (SEC) is the standard method for aggregate and fragment analysis of monoclonal antibodies in biopharmaceutical QC. This application note introduces a new series of silica-based SEC columns engineered to provide shorter analysis time or higher resolution than standard columns for the separation of fragments, monomers and dimers. [![Download Application >>](https://no-cache.hubspot.com/cta/default/2322077/120e1e1b-19ce-48c6-88a4-01c996461ef4.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/120e1e1b-19ce-48c6-88a4-01c996461ef4) ### Application Note Increased Resolution mAb Analysis by UHP-SEC ![Tosoh_App2.png](https://learning.sepscience.com/hs-fs/hubfs/Learning%20Portals/Tosoh/Tosoh_App2.png?width=120&name=Tosoh_App2.png "Tosoh_App2.png")The antibody therapeutics market is enjoying high growth rates, the major areas of therapeutic application being cancer and immune/inflammation-related disorders including arthritis and multiple sclerosis. The standard method used in biopharmaceutical QC for mAb aggregate and fragment analysis is size exclusion chromatography (SEC). This application note introduces the TSKgel UP-SW3000, 2 μm silica-based UHPLC/HPLC columns offering increased speed and higher resolution for the separation of antibody fragments, monomers and dimers. [![Download Application >>](https://no-cache.hubspot.com/cta/default/2322077/120e1e1b-19ce-48c6-88a4-01c996461ef4.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/120e1e1b-19ce-48c6-88a4-01c996461ef4) ### NUCLEIC ACIDS Application Note Analysis of Nucleobases using SEC Columns in HILIC Mode ![Tosoh_App3.png](https://learning.sepscience.com/hs-fs/hubfs/Learning%20Portals/Tosoh/Tosoh_App3.png?width=120&name=Tosoh_App3.png "Tosoh_App3.png")Hydrophilic interaction liquid chromatography (HILIC) is one of the fastest growing modes of separation, in which any polar chromatographic surface can be used. Chemically-bonded diol-coated phases, as found in TSKgel SW size exclusion chromatography (SEC) columns, demonstrate high polarity and hydrogen bonding properties and do not contain ionizable groups other than the unreacted residual silanols, making them appropriate for HILIC mode. This application note introduces the TSKgel SuperSW mAb HTP column and demonstrates the benefits of using it in HILIC mode for the superior resolution of four nucleobases, as opposed to using the column in SEC mode or using a HILIC column. [![Download Application >>](https://no-cache.hubspot.com/cta/default/2322077/120e1e1b-19ce-48c6-88a4-01c996461ef4.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/120e1e1b-19ce-48c6-88a4-01c996461ef4) ### RECOMBINANT PROTEINS Application Note Smart Solutions for Large Molecules using SEC , IEC and HIC ![Tosoh_App4.png](https://learning.sepscience.com/hs-fs/hubfs/Learning%20Portals/Tosoh/Tosoh_App4.png?width=120&name=Tosoh_App4.png "Tosoh_App4.png")Development and production of biopharmaceuticals is a growing segment of pharmaceutical industry. A thorough characterization of therapeutic biomolecules is a key task for the successful submission of research and production data for regulatory approvals of new drugs. Due to its high degree of automation, robustness and reproducibility liquid chromatography is the workhorse of the pharmaceutical laboratory. Therefore, HPLC is also frequently used when establishing analysis methods for biotherapeutics. This application note features some of the most frequently used HPLC applications for the characterization of biopharmaceuticals. [![Download Application >>](https://no-cache.hubspot.com/cta/default/2322077/120e1e1b-19ce-48c6-88a4-01c996461ef4.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/120e1e1b-19ce-48c6-88a4-01c996461ef4) ### Application Note Characterization of PEGylated Lysozyme by IEC AND SEC ![Tosoh_App5.png](https://learning.sepscience.com/hs-fs/hubfs/Learning%20Portals/Tosoh/Tosoh_App5.png?width=120&name=Tosoh_App5.png "Tosoh_App5.png")PEGylation, the process by which polyethylene glycol (PEG) chains are attached to protein and peptide drugs is a common practice in the development of biopharmaceuticals to prolong serum half-life and improve pharmacokinetics of a drug. There is increasing demand for chromatographic methods to separate the modified isoforms from the native protein. This application note describes the use of size exclusion and ion exchange chromatography for the characterization of PEGylated lysozyme. [![Download Application >>](https://no-cache.hubspot.com/cta/default/2322077/120e1e1b-19ce-48c6-88a4-01c996461ef4.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/120e1e1b-19ce-48c6-88a4-01c996461ef4) ### TIPS AND TRICKS Application Note Method Transfer from HPLC to UHPLC for Biomolecules ![Tosoh_App6.png](https://learning.sepscience.com/hs-fs/hubfs/Learning%20Portals/Tosoh/Tosoh_App6.png?width=120&name=Tosoh_App6.png "Tosoh_App6.png")The use of UHPLC systems for small molecule analysis has gained widespread acceptance among researchers in recent years. The use of HPLC columns with UHPLC systems offers the advantages of cost and time savings over having to purchase and develop new methods with UHPLC columns. This application note demonstrates the excellent performance of conventional TSKgel HPLC columns on a UHPLC system. [![Download Application >>](https://no-cache.hubspot.com/cta/default/2322077/120e1e1b-19ce-48c6-88a4-01c996461ef4.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/120e1e1b-19ce-48c6-88a4-01c996461ef4)      Eclipse Business Media Ltd, Regd in England, No. 06513189, Woodview, Bull Lane Industrial Estate, Sudbury, CO10 0FD, United Kingdom, T +44 (0)161 818 7434, [info@sepscience.com](mailto:info@sepscience.com)   Eclipse Business Media Ltd needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our [Privacy Policy](https://www.sepscience.com/privacy-policy/) or contact [privacy@labxmediagroup.com](mailto:privacy@labxmediagroup.com)  Copyright © 2025. 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