BioPharmaceutical Characterization Resource Center

Thermo Scientific SMART Digest Kit

Obtain high quality analytical results from protein digests by using Thermo Scientific™ SMART Digest™ Kits. A significant advance in sample preparation for biopharmaceutical and protein research, the kits provide fast and simple protein digestion with high reproducibility, high sensitivity, and high levels of data quality in a format that's compatible with automation. The SMART Digest Kits are a significant improvement over current in-solution protein digestion technologies, which are not reproducible, have poor sensitivity, and employ protracted methodologies not amenable to automation.]

Take the SMART Route to Protein Digestion

In part 1 of this peptide mapping webinar series join Merck and to learn how to digest biotherapeutic proteins more efficiently & reproducibly. Peptide mapping is an essential step in biotherapeutic drug characterization. In this series 4 new technologies will be demonstrated that dramatically improve upon established mapping workflows. Upgrade your maps! Learn how to increase confidence in biopharma peptide mapping.

Peptide Separations with Pinpoint Precision

In part 2 of this webinar series join NIBRT and learn how cutting edge UHPLC provides the ultimate retention time reproducibility and high resolution separation of peptides. Peptide mapping is an essential step in biotherapeutic drug characterization. In this series 4 new technologies will be demonstrated that dramatically improve upon established mapping workflows. Upgrade your maps! Learn how to increase confidence in biopharma peptide mapping.

Don’t Miss a Thing on Your Peptide Mapping Journey

In part 3 of this peptide mapping webinar series see how easy it is to achieve full coverage peptide maps with Orbitrap-based high resolution accurate mass (HRAM) mass spectrometry (MS). Peptide mapping is an essential step in biotherapeutic drug characterization. In this series 4 new technologies will be demonstrated that dramatically improve upon established mapping workflows. Upgrade your maps! Learn how to increase confidence in biopharma peptide mapping.

High-precision, Automated Peptide Mapping of Proteins

Hear Dr. Amy Farrell of the National Institute of Bioprocessing Research and Training (NIBRT) discuss the development of a robust and reproducible, high-precision, automated, protein digestion workflow that is appropriate as an easy-to-use, general approach to peptide mapping characterization with both LC-only and LC-MS processes. The quality of peptide maps was confirmed with multiple example proteins and high sequence coverage was possible using high-resolution, accurate-mass mass spectrometry.

[eBook] Methods & Applications in BioPharmaceutical Characterization

Read about...

Intact Protein Analysis
Monoclonal Subunit Mass Analysis
Peptide Mapping Analysis
Peptide Mapping
Labeled Glycan Analysis
Unlabeled Glycan Analysis
Aggregate Analysis
Charge Variant Analysis

Tandem UHPLC operation for high-throughput LC-MS peptide mapping analyses

The aim of this work was to demonstrate the use of the new Thermo Scientific™ Vanquish™ Duo UHPLC system and enable tandem analysis with two columns in parallel, addressing productivity and throughput improvement of existing LC-MS methods.

SMART Digest compared to classic in-solution digestion of rituximab for in-depth peptide mapping characterization

To compare the results achieved by using the newly developed Thermo Scientific™ SMART Digest™ kit to those obtained from classic in-solution protein digestion methods, focusing on protein sequence coverage and identified post-translational modifications (PTMs), including deamidation, oxidation, and glycosylation.

Complete characterization of a lysine-linked antibody drug conjugate by native LC/MS intact mass analysis and peptide mapping

To perform complete characterization of the lysine-linked antibody drug conjugate (ADC) trastuzumab emtansine using both intact mass and peptide mapping approaches with a single benchtop mass spectrometer. Critical quality attribute (CQA) measurements include drug-to-antibody ratio (DAR) and glycosylation pattern via intact mass analysis, and conjugation site localization via peptide mapping.

Upgrade Your Maps - Fast, Confident and More Reliable Peptide Mapping

Peptide mapping is a core analytic in biotherapeutic development. Yet it is slow, involves painstaking sample preparation, and is prone to errors. This booklet will guide you through the rough terrain of peptide mapping and upgrade you with a solution that’s better, wherever on the map you are.