Instrument configuration for native N-linked oligosaccharide characterization by HPAE-PAD/MS
Provide application installation instructions for coupling HPAE-PAD analyses to the Thermo Scientific™ Q Exactive™ family of mass spectrometers. This technical note focuses on using this set up to profile N-linked glycans released from glycoproteins.
Preparation of peptide N-Glycosidase F digests for HPAE-PAD analysis
The work shown here describes glycoprotein deglycosylation with PNGase F, followed by an efficient sample pretreatment using desalting and detergent removal spin columns to remove high concentrations of salt and detergents from small-scale PNGase F digestions. The HPAE-PAD technique was used to separate and detect the oligosaccharides.
Label-free Profiling of O-linked Glycans